Composite
Part:BBa_K3583009:Design
Designed by: Sebastian Kusnik Group: iGEM20_Duesseldorf (2020-10-22)
Physcomitrella patens PPK with vacuolar import peptide
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 3099
Illegal XbaI site found at 75
Illegal XbaI site found at 146
Illegal XbaI site found at 2113
Illegal XbaI site found at 2191 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 3099
Illegal NheI site found at 121
Illegal NheI site found at 168 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 3099
Illegal BglII site found at 2839
Illegal BamHI site found at 353
Illegal BamHI site found at 1073
Illegal XhoI site found at 348 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 3099
Illegal XbaI site found at 75
Illegal XbaI site found at 146
Illegal XbaI site found at 2113
Illegal XbaI site found at 2191 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 3099
Illegal XbaI site found at 75
Illegal XbaI site found at 146
Illegal XbaI site found at 2113
Illegal XbaI site found at 2191
Illegal NgoMIV site found at 770 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3346
Illegal SapI site found at 3278
Illegal SapI.rc site found at 1178
Illegal SapI.rc site found at 1724
Illegal SapI.rc site found at 1933
Design Notes
The stop codon of the enzyme has to be moved behind the PSI.
Source
Check the pages of each Biobrick.